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【 results 】 purification later got a strain gram-negative, no spores sindbis purebred bacteria, sequencing the after 1477bp 16S rDNA fragment length for bacterial strain, and pseudomonas, the closest of paternity 16S rDNA genetic similarity over 99%, preliminary determine its for pseudomonas genera. 【 conclusion 】 16S rDNA method can be applied quickly and trace, accurate, easy to determine the bacteria, classification and identification of activated sludge containing pseudomonas genera of the bacteria, this experiment as we optimized active sludge strains, so as to raise the ability of activated sludge treatment sludge and improve the process laid a foundation.